Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010721
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dc.contributor.authorMAIA, M. da S.pt_BR
dc.contributor.authorBICUDO, S. D.pt_BR
dc.contributor.authorRODELLO, L.pt_BR
dc.date.accessioned2015-03-05T11:11:11Zpt_BR
dc.date.available2015-03-05T11:11:11Zpt_BR
dc.date.created2015-03-05pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationAnimal Reproduction, v. 11, n. 2, p. 119-123, apr../jun. 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1010721pt_BR
dc.descriptionOxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37°C with 100 ?M H2O2, 1 U catalase + 100 ?M H2O2 or no treatment, for 30 min. Immediately after adding treatments, sperm motility was determined by computer-assisted semen analysis (CASA). Incubation with H2O2 led to a significant (P < 0.05) decrease in motility parameters whereas catalase prevented a decline in motility secondary to oxidative stress. After 30 min of incubation with H2O2, total motility (12.0% vs. control 73.0%, H2O2 +CAT 70.0%), progressive motility (0.0% vs. control 19.0%, H2O2 +CAT 19.0%) and rapid motility (1.0% vs. control 43.0%, H2O2 +CAT 40.0%) decreased significantly (P < 0.05), whereas percentage of static cells increased (84.0% vs. control 18.0%, H2O2 +CAT 20.0%). We conclude that H2O2 causes damage to ovine sperm motility and that catalase is able to avoid detrimental effect of H2O2 on sperm motility.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEstresse oxidativopt_BR
dc.subjectAntioxidantespt_BR
dc.titleEffect of hydrogen peroxide on thawed ovine sperm motility.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-03-05T11:11:11Zpt_BR
dc.subject.thesagroProdução animalpt_BR
dc.subject.thesagroOvinopt_BR
dc.subject.thesagroCatalasept_BR
dc.subject.thesagroDescongelamentopt_BR
dc.subject.thesagroSêmenpt_BR
dc.subject.nalthesaurusAnimal productionpt_BR
riaa.ainfo.id1010721pt_BR
riaa.ainfo.lastupdate2015-03-05pt_BR
dc.contributor.institutionMARCIANE DA SILVA MAIA, CPATSA; S. D. BICUDO, São Paulo State University; L. RODELLO, São Paulo State University.pt_BR
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