Título:	Identification and characterization of microRNAs expressed in chicken skeletal muscle.
Autoria:	ANDREOTE, A. P. D. ROSÁRIO, M. F. do LEDUR, M. C. JORGE, E. C. SONSTEGARD, T. S. MATUKUMALLI, L. COUTINHO, L. L.
Afiliação:	ANA PAULA DINI ANDREOTE, CENA/USP; MILLOR FERNANDES DO ROSÁRIO, ESALQ; MONICA CORREA LEDUR, CNPSA; ERIKA CRISTINA JORGE, CENA/USP; Animal Research Center/USDA; Animal Research Center/USDA; LUIS LEHMANN COUTINHO, ESALQ.
Ano de publicação:	2014
Referência:	Genetics and Molecular Research, v. 6, n. 1, p. 1465-1479, 2014.
Conteúdo:	MicroRNAs (miRNAs, miRs) encompass a class of small non-coding RNAs that often negatively regulate gene expression. miRNAs play an essential role in skeletal muscle, determining the proper development and maintenance of this tissue. In comparison to other organs and tissues, the full set of muscle miRNAs and its expression patterns are still poorly understood. In this report, a chicken skeletal muscle miRNA library was constructed, and the expression of selected miRNAs was further characterized during muscle development in chicken lines with distinct muscling phenotypes. Clone library sequence analysis revealed 40 small RNAs with similarities to previously described chicken miRNAs, seven miRNAs that were never identified before in chicken, and some sequence clusters representing other possible novel miRNAs. Temporal expression profiles of three miRNAs associated with cell proliferation and differentiation (miR-125b, miR-221, and miR-206) in two chicken lines (broiler and layer) revealed the differential steady-state levels of these miRs during skeletal muscle growth and suggests that miR-206 is involved in the muscling phenotype that is observed in growth-selected chicken lines.
Thesagro:	Genetica animal Galinha
NAL Thesaurus:	Chickens Molecular genetics Cell proliferation
Digital Object Identifier:	10.4238/2014.March.6.5.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPSA)