Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1019494
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dc.contributor.authorPINTO, M. dos S. T.pt_BR
dc.contributor.authorRIBEIRO, J. M.pt_BR
dc.contributor.authorARAUJO, F. P. dept_BR
dc.contributor.authorMELO, N. F. dept_BR
dc.contributor.authorFERNANDES, K. V. S.pt_BR
dc.date.accessioned2017-05-03T23:47:05Z-
dc.date.available2017-05-03T23:47:05Z-
dc.date.created2015-07-08pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationAfrican Journal of Biotechnology, v. 14, n. 21, p. 1838-1845, 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1019494pt_BR
dc.descriptionUmbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 7 0°C for 6 min. POX activity present in crude extracts was more heat - resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azid e (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectSpondia tuberosapt_BR
dc.subjectGuaiacol peroxidasept_BR
dc.subjectRoot enzymept_BR
dc.subjectTssue brownigpt_BR
dc.titlePurification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-05-03T23:47:05Zpt_BR
dc.subject.thesagroUmbupt_BR
dc.subject.thesagroCaatingapt_BR
dc.subject.thesagroPeroxidasept_BR
riaa.ainfo.id1019494pt_BR
riaa.ainfo.lastupdate2017-05-03pt_BR
dc.identifier.doi10.5897/AJB2015.14521pt_BR
dc.contributor.institutionMARCIO DOS SANTOS TEIXEIRA PINTO, Universidade Federal do Tocantins - UFT; JULIANA MARTINS RIBEIRO, CPATSA; FRANCISCO PINHEIRO DE ARAUJO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA; KÁTIA VALEVSKI SALES FERNANDES, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF.pt_BR
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