Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032210
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dc.contributor.authorMACHADO-DE-ÁVILA, R. A.pt_BR
dc.contributor.authorVELLOSO, M.pt_BR
dc.contributor.authorOLIVEIRA, D.pt_BR
dc.contributor.authorSTRANSKY, S.pt_BR
dc.contributor.authorFLOR-SÁ, A.pt_BR
dc.contributor.authorSCHNEIDER, F. S.pt_BR
dc.contributor.authorNESHICH, G.pt_BR
dc.contributor.authorCHÁVEZ-OLÓRTEGUI, C.pt_BR
dc.date.accessioned2015-12-22T11:11:11Zpt_BR
dc.date.available2015-12-22T11:11:11Zpt_BR
dc.date.created2015-12-22pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationImmunome Research, v. 11, n. 1, Mar. 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1032210pt_BR
dc.descriptionMutalysin-II, from Lachesis muta muta snake venom, is an endopeptidase with hemorrhagic activity. To identify a conformational epitope we used Blue Star Sting, the latest version of the web based Sting Millenium Suite, as alternative computational analysis tool to select and design peptides. Pre-selected cut-off values of the accessibility and hydrophilicity parameters were used to select amino acid residues as potential conformational epitopes. A peptide (P117-Y116-C115-Q194-C195-L197-N198-K199-P200-Y5-L48) was manually drawn on Swiss-PDB-Viewer package and synthesized by Fmoc-synthesis. Immunization of rabbits with this peptide induced antibodies that recognized Mutalysin-II and protected against the hemorrhagic factors present in Lachesis venom. The Sting Millennium Suite was able to predict conformational epitopes in this class of proteins. Three amino acids (K199, Y5 and L48) were identified as essential in the interaction between the peptide and the neutralizing antibodies.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPeptídeospt_BR
dc.subjectSting millenniumpt_BR
dc.subjectConformational epitope predictionpt_BR
dc.titleInduction of neutralizing antibodies against mutalysin-II from Lachesis muta muta Snake Venom Elicited by a conformational B-cell epitope predicted by Blue Star Sting data base.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-12-22T11:11:11Zpt_BR
dc.subject.thesagroVenenopt_BR
dc.subject.nalthesaurusLachesis mutapt_BR
dc.subject.nalthesaurusAntivenomspt_BR
dc.subject.nalthesaurusSynthetic peptidespt_BR
dc.subject.nalthesaurusEpitopespt_BR
dc.format.extent2Não paginado.pt_BR
riaa.ainfo.id1032210pt_BR
riaa.ainfo.lastupdate2015-12-22pt_BR
dc.identifier.doihttp://dx.doi.org/10.4172/1745-7580.1000083pt_BR
dc.contributor.institutionRICARDO ANDREZ MACHADO-DE-ÁVILA, Universidade do Extremo Sul Catarinense, Criciúma-SC; MARIANA VELLOSO, UFMG; DAYSIANE OLIVEIRA, UFMG; STEPHANIE STRANSKY, UFMG; ANA FLOR-SÁ, UFMG; FRANCISCO S. SCHNEIDER, UFMG; GORAN NESHICH, CNPTIA; CARLOS CHÁVEZ-OLÓRTEGUI, Universidade do Extremo Sul Catarinense, Criciúma-SC.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPTIA)

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