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dc.contributor.authorPÉREZ, E.pt_BR
dc.contributor.authorRUBIO, M. B.pt_BR
dc.contributor.authorCARDOZA, R. E.pt_BR
dc.contributor.authorGUTIÉRREZ, S.pt_BR
dc.contributor.authorBETTIOL, W.pt_BR
dc.contributor.authorMONTE, E.pt_BR
dc.contributor.authorHERMOSA, R.pt_BR
dc.date.accessioned2016-01-04T11:11:11Zpt_BR
dc.date.available2016-01-04T11:11:11Zpt_BR
dc.date.created2016-01-04pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationFrontiers in Microbiology, Lausanne, v. 6, 2015. Article 1181.pt_BR
dc.identifier.isbnhttp://dx.doi.org/10.3389/fmicb.2015.01181pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1032746pt_BR
dc.descriptionSpecies of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato plant responses was examined in greenhouse assays. The growth of plants colonized by the silenced transformants was reduced and the plants exhibited an increased susceptibility to B. cinerea in comparison with the responses observed for control plants. In addition, the plants turned yellowish and were defective in jasmonic acid- and ethylene-regulated signaling pathways which was seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectTparo7 genept_BR
dc.subject2-phenylethanolpt_BR
dc.subjectTyrosolpt_BR
dc.titleThe importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-01-25T11:11:11Zpt_BR
dc.subject.thesagroTrichodermapt_BR
dc.subject.thesagroControle biológicopt_BR
dc.subject.nalthesaurusShikimate pathwaypt_BR
dc.subject.nalthesaurusGene silencingpt_BR
dc.subject.nalthesaurusAntifungal agentspt_BR
dc.subject.nalthesaurusPhenylethyl alcoholpt_BR
dc.subject.nalthesaurusSalicylic acidpt_BR
dc.format.extent214 p.pt_BR
riaa.ainfo.id1032746pt_BR
riaa.ainfo.lastupdate2016-01-25pt_BR
dc.contributor.institutionESCLAUDYS PEREZ, Universidad de Salamanca; MARIA BELEN RUBIO, Universidad de Salamanca; ROSA ELENA CARDOSA, Universidad de León; SANTIAGO GUTIERREZ, Universidad de León; WAGNER BETTIOL, CNPMA; ENRIQUE MONTE, Universidad de Salamanca; ROSA HERMOSA, Universidad de Salamanca.pt_BR
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