Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1033914
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dc.contributor.authorSANTOS, M. R. A. dospt_BR
dc.contributor.authorCHAGAS, S. E. da S.pt_BR
dc.contributor.authorGUIMARÃES, M. de C. M.pt_BR
dc.date.accessioned2016-01-14T11:11:11Zpt_BR
dc.date.available2016-01-14T11:11:11Zpt_BR
dc.date.created2016-01-14pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationSaber Científico, Porto Velho, v. 4, n. 2, p. 15-23, 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1033914pt_BR
dc.descriptionThe objective of this work was the establishment of an efficient protocol for surface sterilization of leaf explants aiming at its introduction in callogenesis and somatic embryogenesis studies. The leaves were washed with distilled water and a detergent agent. In a laminar flow hood they were immersed in alcohol 70% (v/v) for 1 minute and then immersed in calcium hypochlorite solutions 0.25, 0.5, 0.75, 1.0, 1.25, 2.5, 5.0 or 10.0% (w/v) during 30 minutes, rinsed three times in distilled water, and cut in 1cm2 fragments, which were inoculated in MS medium. Half of the explants were inoculated with the abaxial epidermis in contact with the medium and half with the adaxial epidermis facedown. Each experimental unit was represented by 10 explants. The cultures were kept in the dark at 24±2ºC. Seven days after inoculation the percentages of contamination and necrosis of the explants were evaluated. The most efficient were the concentrations of 0.25, 0.5 and 0.75% calcium hypochlorite, which resulted in total sterilization of the explants. Treatments with 1.0, 1.25, 2.5, 5.0 and 10.0% calcium hypochlorite resulted in high level of necrosis (100%), independent of the side in contact with the medium. All the abaxial side facedown explants kept green and the adaxial ones became necrotic. It is recommendable 0.25% calcium hypochlorite in the surface sterilization and the abaxial epidermis in contact with the culture medium.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCultura de tecidos vegetaispt_BR
dc.subjectHipoclorito de cálciopt_BR
dc.subjectEstabelecimento in vitropt_BR
dc.subjectDescontaminação de explantespt_BR
dc.subjectPlant tissue culturept_BR
dc.subjectIn vitro establishmentpt_BR
dc.subjectExplant decontaminationpt_BR
dc.titleEstablishment of protocol for decontamination of leaf explants of Platonia insignis Mart.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-01-14T11:11:11Zpt_BR
dc.subject.thesagroPlatonia Insignispt_BR
dc.subject.nalthesauruscalcium hypochloritept_BR
riaa.ainfo.id1033914pt_BR
riaa.ainfo.lastupdate2016-01-14pt_BR
dc.contributor.institutionMAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Sâmela Emanuela da Silva Chagas, Faculdade São Lucas; Milene de Castro Melo Guimarães, Mestranda, Unir.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CPAF-RO)

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