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dc.contributor.authorROSSONI, R. D.pt_BR
dc.contributor.authorBARBOSA, J. O.pt_BR
dc.contributor.authorVILELA, S. F. G.pt_BR
dc.contributor.authorSANTOS, J. D. dospt_BR
dc.contributor.authorBARROS, P. P. dept_BR
dc.contributor.authorPRATA, M. C. de A.pt_BR
dc.contributor.authorANBINDER, A. L.pt_BR
dc.contributor.authorFUCHS, B. B.pt_BR
dc.contributor.authorJORGE, A. O. C.pt_BR
dc.contributor.authorMYLONAKIS, E.pt_BR
dc.contributor.authorJUNQUEIRA, J. C.pt_BR
dc.date.accessioned2016-01-25T11:11:11Zpt_BR
dc.date.available2016-01-25T11:11:11Zpt_BR
dc.date.created2016-01-25pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationPLoS One, v. 10, n. 7, 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1035034pt_BR
dc.descriptionIn this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectAnimal modelpt_BR
dc.titleCompetitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-14T11:11:11Zpt_BR
dc.subject.nalthesaurusGalleria mellonellapt_BR
dc.subject.nalthesaurusinfectionpt_BR
dc.format.extent223 p.pt_BR
riaa.ainfo.id1035034pt_BR
riaa.ainfo.lastupdate2016-03-14pt_BR
dc.contributor.institutionRodnei Dennis Rossoni; Júnia Oliveira Barbosa; Simone Furgeri Godinho Vilela; Jéssica Diane dos Santos; Patrícia Pimentel de Barros; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Ana Lia Anbinder; Beth Burgwyn Fuchs; Antonio Olavo Cardoso Jorge; Eleftherios Mylonakis; Juliana Campos Junqueira.pt_BR
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