Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1046809
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dc.contributor.authorARDISSON-ARAÚJO, D. M. P.pt_BR
dc.contributor.authorLIMA, R. N.pt_BR
dc.contributor.authorMELO, F. L.pt_BR
dc.contributor.authorCLEM, R. J.pt_BR
dc.contributor.authorHUANG, N.pt_BR
dc.contributor.authorBÁO, S. N.pt_BR
dc.contributor.authorSOSA-GÓMEZ, D. R.pt_BR
dc.contributor.authorRIBEIRO, B. M.pt_BR
dc.date.accessioned2017-06-19T23:56:22Z-
dc.date.available2017-06-19T23:56:22Z-
dc.date.created2016-06-13pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationScientific Reports, v. 6, n. 24612, jun. 2016.pt_BR
dc.identifier.issn2045-2322pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1046809pt_BR
dc.descriptionThe genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (pelu112). The pelu112 gene was predicted to encode a fusion of thymidylate kinase (tmk) and dUTP diphosphatase (dut). Phylogenetic analysis indicated that baculoviruses have independently acquired tmk and dut several times during their evolution. Two homologs of the tmk-dut fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks tmk and dut. The recombinant baculoviruses produced viral DNA, virus progeny, and some viral proteins earlier during in vitro infection and the yields of viral occlusion bodies were increased 2.5-fold when compared to the parental virus. Interestingly, both enzymes appear to retain their active sites, based on separate modeling using previously solved crystal structures. We suggest that the retention of these tmk-dut fusion genes by certain baculoviruses could be related to accelerating virus replication and to protecting the virus genome from deleterious mutation.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleGenome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-06-19T23:56:22Zpt_BR
dc.subject.thesagroBaculoviruspt_BR
dc.subject.thesagroViruspt_BR
dc.subject.thesagroGenomapt_BR
dc.subject.nalthesaurusBaculoviridaept_BR
dc.subject.nalthesaurusGenome assemblypt_BR
riaa.ainfo.id1046809pt_BR
riaa.ainfo.lastupdate2017-06-19pt_BR
dc.identifier.doi10.1038/srep24612pt_BR
dc.contributor.institutionDANIEL M. P. ARDISSON-ARAÚJO, UNB; RAYANE NUNES LIMA, UNB; FERNANDO L. MELO, UNB; ROLLIE J. CLEM, KANSAS STATE UNIVERSITY; NING HUANG, Kansas State University; SÔNIA NAIR BÁO, UNB; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN M. RIBEIRO, UNB.pt_BR
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