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dc.contributor.authorCARVALHO, L. J. C. B.pt_BR
dc.contributor.authorAGUSTINI, M. A. V.pt_BR
dc.contributor.authorANDERSON, J. V.pt_BR
dc.contributor.authorVIEIRA, E. A.pt_BR
dc.contributor.authorSOUZA, C. R. B. dept_BR
dc.contributor.authorCHEN, S.pt_BR
dc.contributor.authorSCHAAL, B. A.pt_BR
dc.contributor.authorSILVA, J. P. dapt_BR
dc.date.accessioned2016-08-23T08:11:43Z-
dc.date.available2016-08-23T08:11:43Z-
dc.date.created2016-08-22pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationBMC Plant Biology, v. 16, n. 1, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1051346pt_BR
dc.descriptionABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total ß-carotene accumulation. CONCLUSIONS: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total ß-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCaretonóidept_BR
dc.subjectHPLC-DADpt_BR
dc.subjectMicromatrizes de DNApt_BR
dc.subjectQRT_PCRpt_BR
dc.titleNatural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-08-23T08:11:43Zpt_BR
dc.subject.thesagroMandiocapt_BR
dc.subject.thesagroArmazenamentopt_BR
dc.subject.nalthesaurusCassavapt_BR
dc.subject.nalthesaurusStoragept_BR
dc.subject.nalthesaurusCarotenoidspt_BR
dc.subject.nalthesaurusDNA microarrayspt_BR
riaa.ainfo.id1051346pt_BR
riaa.ainfo.lastupdate2016-08-22pt_BR
dc.identifier.doi10.1186/s12870-016-0826-0pt_BR
dc.contributor.institutionLUIZ JOAQUIM CASTELO B CARVALHO, Cenargen; MARCO A. V. AGUSTINI, Cenargen; JAMES V. ANDERSON, USDA ARS; EDUARDO ALANO VIEIRA, CPAC; CLAUDIA R. B. DE SOUZA, UFPA; SONGBI CHEN, Chinese Acad Trop Agr, Trop Crops Genet Resources Inst; BARBARA A. SCHAAL, Washington Univ; JOSEANE PADILHA DA SILVA, Cenargen.pt_BR
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