Título:	Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.
Autoria:	MOREIRA, C. M. ANDRADE, H. B. de BERTOLUCCI, S. K. V. LAMEIRA, O. A. MOHAMMED, A. PINTO, J. E. B. P.
Afiliação:	Carolina Mariane MOREIRA, UFLA; Helena Botelho de ANDRADE, UFLA; Suzan Kelly Vilela BERTOLUCCI, UFLA; OSMAR ALVES LAMEIRA, CPATU; Aliyu MOHAMMED, Abant ?zzet Baysal University; José Eduardo Brasil Pereira PINTO, UFLA.
Ano de publicação:	2016
Referência:	Turkish Journal of Biology, v. 40, n. 6, p. 1227-1234, 2016.
Conteúdo:	The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.
Thesagro:	Curaua Regeneração
NAL Thesaurus:	thidiazuron
Palavras-chave:	Regeneração in vitro
Digital Object Identifier:	10.3906/biy-1601-56
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATU)