Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060930
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dc.contributor.authorBAPTISTA, R. C.pt_BR
dc.contributor.authorFERNANDES, M. A. M.pt_BR
dc.contributor.authorGILAVERTE, S.pt_BR
dc.contributor.authorQUEIROZ, S. C. do N. dept_BR
dc.contributor.authorASSALIN, M. R.pt_BR
dc.contributor.authorFERRACINI, V. L.pt_BR
dc.contributor.authorMONTEIRO, A. L. G.pt_BR
dc.contributor.authorREYES, F. G. R.pt_BR
dc.date.accessioned2017-01-17T11:11:11Zpt_BR
dc.date.available2017-01-17T11:11:11Zpt_BR
dc.date.created2017-01-17pt_BR
dc.date.issued2017pt_BR
dc.identifier.citationJournal of the Brazilian Chemical Society, Campinas, v. 28, n. 2, p. 250-256, 2017.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1060930pt_BR
dc.descriptionAbstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectSerum lambpt_BR
dc.subjectLC-MS/MSpt_BR
dc.titleDetermination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-02T11:11:11Zpt_BR
dc.subject.thesagroCordeiropt_BR
dc.subject.thesagroAnálise de laboratóriopt_BR
dc.subject.thesagroAnti-helmínticopt_BR
dc.subject.thesagroCromatografiapt_BR
dc.subject.thesagroEspectrometriapt_BR
dc.subject.thesagroSoro Sangüíneoeng
dc.subject.nalthesaurusBlood serumpt_BR
dc.subject.nalthesaurusLambspt_BR
dc.subject.nalthesaurusMoxidectinpt_BR
dc.subject.nalthesaurusAnthelminticspt_BR
dc.subject.nalthesaurusPharmacokineticspt_BR
dc.subject.nalthesaurusVeterinary drugspt_BR
dc.subject.nalthesaurusTandem mass spectrometrypt_BR
dc.subject.nalthesaurusLiquid chromatographypt_BR
riaa.ainfo.id1060930pt_BR
riaa.ainfo.lastupdate2017-03-02pt_BR
dc.contributor.institutionRAFAELA DE CARVALHO BAPTISTA, FEA-UNICAMP; MARIA ANGELA MACHADO FERNANDES, LAPOC-UFPR; SUSANA GILAVERTE, LAPOC-UFPR; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; MARCIA REGINA ASSALIN, CNPMA; VERA LUCIA FERRACINI, CNPMA; ALDA LUCIA GOMES MONTEIRO, LAPOC-UFPR; FELIX GUILLERMO REYES REYES, FEA-UNICAMP.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMA)

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