Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061179
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dc.contributor.authorCARVALHO, R. C. T.pt_BR
dc.contributor.authorVASCONCELLOS, S. E. G.pt_BR
dc.contributor.authorISSA, M. de A.pt_BR
dc.contributor.authorSOARES FILHO, P. M.pt_BR
dc.contributor.authorMOTA, P. M. P. C.pt_BR
dc.contributor.authorARAUJO, F. R.pt_BR
dc.contributor.authorCARVALHO, A. C. da S.pt_BR
dc.contributor.authorGOMES, H. M.pt_BR
dc.contributor.authorSUFFYS, P. N.pt_BR
dc.contributor.authorFIGUEIREDO, E. E. de S.pt_BR
dc.contributor.authorPASCHOALIN, V. M. F.pt_BR
dc.date.accessioned2017-01-19T11:11:11Zpt_BR
dc.date.available2017-01-19T11:11:11Zpt_BR
dc.date.created2017-01-19pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationPlOs ONE, v. 11, n. 9, p. 1-16, Sept. 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1061179pt_BR
dc.descriptionMycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRUVNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = > 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions,municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleMolecular typing of mycobacterium bovis from cattle reared in Midwest Brazil.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-01-19T11:11:11Zpt_BR
dc.subject.thesagroMycobacterium Bovispt_BR
dc.subject.nalthesaurusBovine tuberculosispt_BR
dc.subject.nalthesaurusMolecular epidemiologypt_BR
dc.subject.nalthesaurusCattlept_BR
dc.subject.nalthesaurusBrazilpt_BR
riaa.ainfo.id1061179pt_BR
riaa.ainfo.lastupdate2017-01-19pt_BR
dc.contributor.institutionRICARDO CÉSAR TAVARES CARVALHO, UFRJ; SIDRA EZIDIO GONÇALVES VASCONCELLOS, FIOCRUZ; MARINA DE AZEVEDO ISSA, LANAGRO/MAPA; PAULO MARTINS SOARES FILHO, LANAGRO/MAPA; PEDRO MOACYR PINTO COELHO MOTA, LANAGRO/MAPA; FLABIO RIBEIRO ARAUJO, CNPGC; ANA CAROLINA DA SILVA CARVALHO, UFRJ/UFMT; HARRISON MAGDINIER GOMES, FIOCRUZ; PHILIP NOEL SUFFYS, FIOCRUZ/LANAGRO/MAPA; EDUARDO EUSTÁQUIO DE SOUZA FIGUEIREDO, UFMT; VÂNIA MARGARET FLOSI PASCHOALIN, UFRJ.pt_BR
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