Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062831
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dc.contributor.authorCHEN, Z.pt_BR
dc.contributor.authorHAGEN, D. E.pt_BR
dc.contributor.authorWANG, J.pt_BR
dc.contributor.authorELSIK, C. G.pt_BR
dc.contributor.authorJI, T.pt_BR
dc.contributor.authorSIQUEIRA, L. G. B.pt_BR
dc.contributor.authorHANSEN, P. J.pt_BR
dc.contributor.authorRIVERA, R. M.pt_BR
dc.date.accessioned2017-02-06T11:11:11Zpt_BR
dc.date.available2017-02-06T11:11:11Zpt_BR
dc.date.created2017-02-06pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationEpigenetics, v. 11, n. 7, p. 501-516, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1062831pt_BR
dc.descriptionAbstract Genomic imprinting is an epigenetic mechanism that leads to parental-allele-specific gene expression. Approximately 150 imprinted genes have been identified in humans and mice but less than 30 have been described as imprinted in cattle. For the purpose of de novo identification of imprinted genes in bovine, we determined global monoallelic gene expression in brain, skeletal muscle, liver, kidney and placenta of day ∼105 Bos taurus indicus × Bos taurus taurus F1 conceptuses using RNA sequencing. To accomplish this, we developed a bioinformatics pipeline to identify parent-specific single nucleotide polymorphism alleles after filtering adenosine to inosine (A-to-I) RNA editing sites. We identified 53 genes subject to monoallelic expression. Twenty three are genes known to be imprinted in the cow and an additional 7 have previously been characterized as imprinted in human and/or mouse that have not been reported as imprinted in cattle. Of the remaining 23 genes, we found that 10 are uncharacterized or unannotated transcripts located in known imprinted clusters, whereas the other 13 genes are distributed throughout the bovine genome and are not close to any known imprinted clusters. To exclude potential cis-eQTL effects on allele expression, we corroborated the parental specificity of monoallelic expression in day 86 Bos taurus taurus × Bos taurus taurus conceptuses and identified 8 novel bovine imprinted genes. Further, we identified 671 candidate A-to-I RNA editing sites and describe random X-inactivation in day 15 bovine extraembryonic membranes. Our results expand the imprinted gene list in bovine and demonstrate that monoallelic gene expression can be the result of cis-eQTL effects.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectA-to-I RNA editingpt_BR
dc.subjectX chromosome inactivationpt_BR
dc.subjectAllele-specific gene expressionpt_BR
dc.subjectNext generation sequencingpt_BR
dc.titleGlobal assessment of imprinted gene expression in the bovine conceptus by next generation sequencing.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-02-15T11:11:11Zpt_BR
dc.subject.nalthesaurusgenomic imprintingpt_BR
riaa.ainfo.id1062831pt_BR
riaa.ainfo.lastupdate2017-02-15pt_BR
dc.contributor.institutionZhiyuan Chen, University of Missouri, Columbia, MO, USA; Darren E. Hagen, University of Missouri, Columbia, MO, USA; Juanbin Wang, University of Missouri, Columbia, MO, USA; Christine G. Elsik, University of Missouri, Columbia, MO, USA; Tieming Ji, University of Missouri, Columbia, MO, USA; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; Peter J. Hansen, University of Florida, Gainesville, FL, USA; Rocio M. Rivera, University of Missouri, Columbia, MO.pt_BR
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