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dc.contributor.authorMUNK, M.pt_BR
dc.contributor.authorLADEIRA, L. O.pt_BR
dc.contributor.authorCARVALHO, B. C. dept_BR
dc.contributor.authorCAMARGO, L. S. de A.pt_BR
dc.contributor.authorRAPOSO, N. R. B.pt_BR
dc.contributor.authorSERAPIÁO, R. V.pt_BR
dc.contributor.authorQUINTAO, C. C. R.pt_BR
dc.contributor.authorSILVA, S. R.pt_BR
dc.contributor.authorSOARES, J. S.pt_BR
dc.contributor.authorJORIO, A.pt_BR
dc.contributor.authorBRANDAO, H. de M.pt_BR
dc.date.accessioned2017-02-16T11:11:11Zpt_BR
dc.date.available2017-02-16T11:11:11Zpt_BR
dc.date.created2017-02-16pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationNature, Scientific Reports, v. 6, article 33588, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1064560pt_BR
dc.descriptionThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGene deliverypt_BR
dc.subjectPellucid zonept_BR
dc.titleEfficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-02T11:11:11Zpt_BR
dc.subject.nalthesaurusin vitro fertilizationpt_BR
riaa.ainfo.id1064560pt_BR
riaa.ainfo.lastupdate2017-03-02pt_BR
dc.contributor.institutionMichele Munk, UFJF; Luiz O. Ladeira, UFMG; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nádia R. B. Raposo, UFJF; Raquel V. Serapião, PESAGRO; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; Saulo R. Silva; Jaqueline S. Soares, UFOP; Ado Jorio, UFMG; HUMBERTO DE MELLO BRANDAO, CNPGL.pt_BR
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