Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1068076
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dc.contributor.authorCLEMENTE, Z.pt_BR
dc.contributor.authorMARTINEZ, D. S. T.pt_BR
dc.contributor.authorOLIVEIRA, C. R. M.pt_BR
dc.contributor.authorMAURER-MORELLI, C. V.pt_BR
dc.contributor.authorNUNES, M. C. S.pt_BR
dc.contributor.authorCASTRO, V. L. S. S. dept_BR
dc.date.accessioned2017-07-10T23:49:27Z-
dc.date.available2017-07-10T23:49:27Z-
dc.date.created2017-04-04pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationIn: WORKSHOP IN ENVIRONMENTAL NANOTECHNOLOGY, 2., 2016. Book of Abstracts... Sorocaba: Unesp, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1068076pt_BR
dc.descriptionGraphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length and yolk sac were measured through a stereomicroscope. The mortality in all groups was below 10% and no malformation was observed. The statistical analysis (Two-way ANOVA followed by Bonferroni test) showed that, independently of HA presence, larvae exposed to GO dispended more time in movement, swam a greater distance, and showed higher swimming velocity than controls. The larvae exposed to GO, with or without HA, were about 3.8% shorter than control. There was no difference among groups related to yolk sac. The effect can be related to GO adsorption to larvae body that can act as tactile stimuli. Our previous study supports the hypothesis that GO can cause impairment in neuromuscular development. Further experiments are being performed to clarify these observations.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNanotoxicologypt_BR
dc.titleZebrafish exposure to graphene oxide is related to behavior changes.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2017-07-10T23:49:27Zpt_BR
dc.subject.nalthesaurusEmbryotoxicitypt_BR
dc.subject.nalthesaurusNanomaterialspt_BR
dc.subject.nalthesaurusEcotoxicologypt_BR
dc.format.extent2p. 27.pt_BR
riaa.ainfo.id1068076pt_BR
riaa.ainfo.lastupdate2017-07-10pt_BR
dc.contributor.institutionZ. CLEMENTE; D. S. T. MARTINEZ; C. R. M. OLIVEIRA; C. V. MAURER-MORELLI; M. C. S. NUNES; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA.pt_BR
Aparece nas coleções:Resumo em anais de congresso (CNPMA)

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