Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071398
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dc.contributor.authorDANTZGER, D. D.pt_BR
dc.contributor.authorJONSSON, C. M.pt_BR
dc.contributor.authorAOYAMA, H.pt_BR
dc.date.accessioned2017-08-01T23:50:33Z-
dc.date.available2017-08-01T23:50:33Z-
dc.date.created2017-06-23pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 45., 2016, Natal. Anais... Natal: Sociedade brasileira de bioquímica e biologia molecular, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1071398pt_BR
dc.descriptionIntroduction. Oreochromis niloticus (Nile tilapia) is the most produced and consumed fish in Brazil. Many fish farming use Diflubenzuron (DFB) as insecticide to control parasites responsible for diseases in these fish. This insecticide degradation generates p-chloroaniline (PCA), a potentially carcinogenic and mutagenic compound. Objectives The objective of this study was to evaluate the enzymes catalase (CAT) and transaminases (TGO and TGP) as water toxicity bioindicators using as experimental model the tilapia fish, exposed for 96 h to different combinatory concentrations of DFB and PCA mixtures, in presence of soil. Material and Methods. Samples of tilapia liver were homogenized and the supernatant was used for the biochemical analyses of enzyme activities and protein concentration. All the enzyme activities were determined by spectrophotometry. Results and Discussion. For catalase, in the presence of soil, 80% decrease was observed for the mixture of 25% DFB (75% PCA) and no significant effects were observed for the mixtures of 50 and 75% DFB, in relation to the control. At the same conditions no significant effects were observed on the transaminase TGO activities for all the mixtures of DFB tested. The TGP activities increased 350%, 80% and 60% for the mixtures of 25, 50 and 75% DFB, respectively, in relation to the control. Conclusions. Catalase and the transaminases TGO and TGP can be used as water toxicity bioindicators for the combinatory mixtures of DFB and PCA. The presence of the soil can dramatically change the activities of the enzymes studied. Finally, besides to serve as an alert, the data obtained in this work can be used to establish limit values for the mixture of these compounds in tropical freshwater bodies.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectP-chloroanilinept_BR
dc.subjectBioindicatorspt_BR
dc.titleAnalysis of enzymes as bioindicators on the combinatory effects of the mixtures of diflubenzuron and p-chloroaniline in tilapia in the presence of soil.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2017-08-01T23:50:33Zpt_BR
dc.subject.thesagroEnzimapt_BR
dc.subject.nalthesaurusDiflubenzuronpt_BR
riaa.ainfo.id1071398pt_BR
riaa.ainfo.lastupdate2017-08-01pt_BR
dc.contributor.institutionD. D. DANTZGER; CLAUDIO MARTIN JONSSON, CNPMA; H. AOYAMA.pt_BR
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