Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073486
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dc.contributor.authorALMEIDA, V. M.pt_BR
dc.contributor.authorLUZ, G. A.pt_BR
dc.contributor.authorMARTINS, P. P.pt_BR
dc.contributor.authorGOMES, M. F. C.pt_BR
dc.contributor.authorCOSTA, M. F.pt_BR
dc.contributor.authorLIMA, P. S. da C.pt_BR
dc.contributor.authorVALENTE, S. E. S.pt_BR
dc.date.accessioned2017-08-02T11:11:11Zpt_BR
dc.date.available2017-08-02T11:11:11Zpt_BR
dc.date.created2017-08-02pt_BR
dc.date.issued2017pt_BR
dc.identifier.citationGenetics and Molecular Research, Ribeirão Preto, v.16, n. 3: gmr16039724, 2017.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1073486pt_BR
dc.descriptionMangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectIsolamento do DNApt_BR
dc.subjectMangabeirapt_BR
dc.subjectReação em cadeia da polimerasept_BR
dc.subjectDNA isolationpt_BR
dc.titleComparison of eight methods to isolate genomic DNA from Hancornia speciosa.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-08-02T11:11:11Zpt_BR
dc.subject.nalthesauruspolymerase chain reactionpt_BR
dc.format.extent27 p.pt_BR
riaa.ainfo.id1073486pt_BR
riaa.ainfo.lastupdate2017-08-02pt_BR
dc.identifier.doihttp://dx.doi.org/10.4238/gmr16039724pt_BR
dc.contributor.institutionUFPI; UFPI; UFPI; UFPI; UFPI; PAULO SARMANHO DA COSTA LIMA, CPAMN; UFPI.pt_BR
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