Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074433
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dc.contributor.authorSARAIVA, H. F. R. de A.
dc.contributor.authorBATISTA, R. I. T. P.
dc.contributor.authorALFRADIQUE, V. A. P.
dc.contributor.authorPINTO, P. H. N.
dc.contributor.authorRIBEIRO, L. S.
dc.contributor.authorOLIVEIRA, C. S.
dc.contributor.authorSOUZA FABJAN, J. M. G. de
dc.contributor.authorCAMARGO, L. S. de A.
dc.contributor.authorFONSECA, J. F. da
dc.contributor.authorBRANDÃO, F. Z.
dc.date.accessioned2017-08-25T19:13:08Z-
dc.date.available2017-08-25T19:13:08Z-
dc.date.created2017-08-24
dc.date.issued2017
dc.identifier.citationAnimal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1074433pt_BR
dc.descriptionEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.".
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectSanta Inês
dc.subjectSurvivalpt_BR
dc.subjectAnimal embryospt_BR
dc.titleL-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos.
dc.typeResumo em anais e proceedings
dc.date.updated2019-09-23T11:11:11Zpt_BR
dc.subject.nalthesaurusSheep
dc.subject.nalthesaurusEwes
dc.subject.nalthesaurusCryopreservationpt_BR
dc.subject.nalthesaurusGene expressionpt_BR
dc.subject.nalthesaurusCarnitinept_BR
dc.subject.nalthesaurusIn vitro culturept_BR
dc.subject.nalthesaurusVitrificationpt_BR
dc.subject.nalthesaurusCell culturept_BR
dc.description.notesProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.
riaa.ainfo.id1074433
riaa.ainfo.lastupdate2019-09-23 -03:00:00
dc.contributor.institutionHelena Fabiana Reis de Almeida Saraiva, Universidade de São Paulo (USA) - Pirassununga, SP, Brasil
dc.contributor.institutionRibrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasilpt_BR
dc.contributor.institutionVivian Angélico Pereira Alfradique, UFF - Niterói, RJ, Brasilpt_BR
dc.contributor.institutionPedro Henrique Nicolau Pinto, UFF - Niterói, RJ, Brasilpt_BR
dc.contributor.institutionLilian Santos Ribeiro, UFF - Niterói, RJ, Brasilpt_BR
dc.contributor.institutionCLARA SLADE OLIVEIRA, CNPGLpt_BR
dc.contributor.institutionJoanna Maria Gonçalves de Souza Fabjan, UFF - Niterói, RJ, Brasilpt_BR
dc.contributor.institutionLUIZ SERGIO DE ALMEIDA CAMARGO, CNPGLpt_BR
dc.contributor.institutionJEFERSON FERREIRA DA FONSECA, CNPCpt_BR
dc.contributor.institutionFelipe Zandonadi Brandão, UFF - Niterói, RJ, Brasil.pt_BR
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