Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079274
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dc.contributor.authorMONTEIRO, J. P.
dc.contributor.authorSANTOS, J. M. L. dos
dc.contributor.authorANDRÉ, W. P. P.
dc.contributor.authorVASCONCELOS, J. F.
dc.contributor.authorFROTA, G. A.
dc.contributor.authorRIBEIRO, W. L. C.
dc.contributor.authorFREITAS, E. P. de
dc.contributor.authorMACEDO, I. T. F.
dc.contributor.authorTEIXEIRA, M.
dc.contributor.authorVIEIRA, L. da S.
dc.contributor.authorBEVILAQUA, C. M. L.
dc.date.accessioned2017-11-09T18:15:27Z-
dc.date.available2017-11-09T18:15:27Z-
dc.date.created2017-11-09
dc.date.issued2017
dc.identifier.citationIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1079274-
dc.descriptionAbstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast detection of levamisole resistance in H. contortus.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectResistance to anthelminticspt_BR
dc.titleMolecular diagnostic for levamisol resistance in Haemonchus contortus.
dc.typeResumo em anais e proceedings
dc.date.updated2019-09-23T11:11:11Zpt_BR
dc.subject.thesagroCaprino
dc.subject.thesagroOvino
dc.subject.thesagroAnti-Helmíntico
dc.subject.thesagroResistência a produtos químicos
dc.subject.thesagroHaemonchus Contortuspt_BR
dc.subject.nalthesaurusSheep
dc.subject.nalthesaurusGoats
dc.subject.nalthesaurusSmall ruminants
dc.subject.nalthesaurusAnthelmintics
dc.subject.nalthesaurusDrug resistance
dc.subject.nalthesaurusLevamisole
dc.subject.nalthesaurusBrazil
dc.description.notesIn conjunction with 53rd MSPTM Annual Conference.
riaa.ainfo.id1079274
riaa.ainfo.lastupdate2019-09-23 -03:00:00
dc.contributor.institutionJOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Pós-graduação - Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) - Sobral, CE, Brazil; Gracielle Araújo Frota, UVA - Sobral, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Edilson Pereira de Freitas; Iara Tersia Freitas Macedo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil.
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