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Campo DCValorIdioma
dc.contributor.authorIVOMOTO, S. T.
dc.contributor.authorSAKURAY, L. M.
dc.contributor.authorFERREIRA, L. P.
dc.contributor.authorKITZBERGER, C. S. G.
dc.contributor.authorSCHOLZ, M. B. S.
dc.contributor.authorPOT, D.
dc.contributor.authorLEROY, T.
dc.contributor.authorVIEIRA, L. G. E.
dc.contributor.authorDOMINGUES, D. S.
dc.contributor.authorPEREIRA, L. F. P.
dc.date.accessioned2017-12-23T23:26:21Z-
dc.date.available2017-12-23T23:26:21Z-
dc.date.created2017-12-21
dc.date.issued2017
dc.identifier.citationPlant Physiology and Biochemistry, v. 111, p. 340-347, February, 2017.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1083355-
dc.descriptionLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCafestol
dc.subjectKahweol
dc.subjectRT-qPCR
dc.titleDiterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development.
dc.typeArtigo de periódico
dc.date.updated2017-12-23T23:26:21Zpt_BR
dc.subject.thesagroCafé
dc.subject.nalthesaurusGene expression
dc.subject.nalthesaurusHigh performance liquid chromatography
riaa.ainfo.id1083355
riaa.ainfo.lastupdate2017-12-21
dc.contributor.institutionSuzana T. Ivamoto, IAPAR/UEL; Leonardo M. Sakuray, IAPAR/UEL; Lucia P. Ferreira, IAPAR; Cíntia S. G. Kitzberger, IAPAR; Maria B. S. Scholz, IAPAR; David Pot, CIRAD; Thierry Leroy, CIRAD; Luiz G. E. Vieira, UNOEST; Douglas S. Domingues, UNESP; LUIZ FILIPE PROTASIO PEREIRA, SAPC.
Aparece nas coleções:Artigo em periódico indexado (SAPC)

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