Título:	Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
Autoria:	OKINO, C. H. MONTASSIER, M. F. de OLIVEIRA, A. P. de MONTASSIER, H. J.
Afiliação:	CINTIA HIROMI OKINO, CPPSE; Maria de Fátima Silva Montassier, UNESP; Andressa Peres de Oliveira, UNESP; Helio José Montassier, UNESP.
Ano de publicação:	2018
Referência:	Veterinary Medical Science, v. 80, p. 725-730, fev. 2018.
Conteúdo:	A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.
Thesagro:	Bronquite
NAL Thesaurus:	Infectious bronchitis Bronchitis Infectious bronchitis virus Birds
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)