Título:	Detection of Co-expressed pathway modules associated with mineral concentration and meat quality in nelore cattle.
Autoria:	DINIZ, W. J. S. MAZZONI, G. COUTINHO BANERJEE, P. GEISTLINGER, L. CESAR, A. S. M. BERTOLINI. F. AFONSO, J. OLIVEIRA, P. S. N. TIZIOTO, P. C. KADARMIDEEN, H. N. REGITANO, L. C. de A.
Afiliação:	Wellison J. S. Diniz, UFSCAR; Gianluca Mazzoni, Technical University of Denmark; Luiz L. Coutinho, USP; Priyanka Banerjee, Technical University of Denmark; Ludwig Geistlinger, Graduate School of Public Health and Health Policy; Aline S. M. Cesar, USP; Francesca Bertolini, Technical University of DenmarK; Juliana Afonso, UFSCAR; Priscila S. N. de Oliveira, Bolsista/Embrapa Pecuária Sudeste; Polyana C. Tizioto, USP; Haja N. Kadarmideen, Technical University of Denmark; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Ano de publicação:	2019
Referência:	Frontiers in genetics, v. 10, n. 210, p. 1-12, 2019.
Conteúdo:	Meat quality is a complex trait that is influenced by genetic and environmental factors, which includes mineral concentration. However, the association between mineral concentration and meat quality, and the specific molecular pathways underlying this association, are not well explored. We therefore analyzed gene expression as measured with RNA-seq in Longissimus thoracis muscle of 194 Nelore steers for association with three meat quality traits (intramuscular fat, meat pH, and tenderness) and the concentration of 13 minerals (Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, and Zn). We identified seven sets of co-expressed genes (modules) associated with at least two traits, which indicates that common pathways influence these traits. From pathway analysis of module hub genes, we further found an over-representation for energy and protein metabolism (AMPK and mTOR signaling pathways) in addition to muscle growth, and protein turnover pathways. Among the identified hub genes FASN, ELOV5, and PDE3B are involved with lipid metabolism and were affected by previously identified eQTLs associated to fat deposition. The reported hub genes and over-represented pathways provide evidence of interplay among gene expression, mineral concentration, and meat quality traits. Future studies investigating the effect of different levels of mineral supplementation in the gene expression and meat quality traits could help us to elucidate the regulatory mechanism by which the genes/pathways are affected.
Thesagro:	Gordura Animal Gado de Corte
NAL Thesaurus:	Intramuscular fat Beef quality Beef cattle
Palavras-chave:	AMPK pathway Co-expression analysis RNA sequencing Tenderness
Digital Object Identifier:	10.3389/fgene.2019.00210
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)