Título:	Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility.
Autoria:	BOTTA, D. ARRUDA, R. P. de WATANABE, Y. F. BALIEIRO, J. C. de C. ROMANELLO, N. BARRETO, A. do N. PANTOJA, M. H. de A. GIRO, A. CARVALHO, C. P. T. de OLIVEIRA, A. de S. GARCIA, A. R.
Afiliação:	Daniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE.
Ano de publicação:	2019
Referência:	Andrologia, v. 51, n. 6, e13266, jul. 2019.
Conteúdo:	Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment &#8805;36.0°C for 4 hr.
Thesagro:	Touro Inseminação Artificial
NAL Thesaurus:	Bulls In vitro fertilization Heat stress
Palavras-chave:	Semen quality Thermotolerance
Digital Object Identifier:	10.1111/and.13266
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)