Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113864
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorSOUZA, A. R. deeng
dc.contributor.authorYAMIN, M.eng
dc.contributor.authorGAVA, D.eng
dc.contributor.authorZANELLA, J. R. C.eng
dc.contributor.authorGATTI, M. S. V.eng
dc.contributor.authorBONAFE, C. S. F.eng
dc.contributor.authorLIMA NETO, D. F. deeng
dc.date.accessioned2019-11-04T18:11:35Z-
dc.date.available2019-11-04T18:11:35Z-
dc.date.created2019-11-04
dc.date.issued2019
dc.identifier.citationVirology Journal, v. 16, art. 75, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1113864-
dc.descriptionAbstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEpitope predictioneng
dc.subjectPorcine parvoviruseng
dc.subjectSpot synthesiseng
dc.titlePorcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-11-04T18:11:35Z
dc.subject.thesagroVirologiaeng
dc.subject.thesagroSuinoculturaeng
dc.subject.thesagroParvoviroseeng
dc.subject.nalthesaurusEpitope mappingeng
dc.subject.nalthesaurusUngulate protoparvovirus 1eng
riaa.ainfo.id1113864eng
riaa.ainfo.lastupdate2019-11-04
dc.identifier.doi10.1186/s12985-019-1165-1eng
dc.contributor.institutionANCELMO RABELO DE SOUZA, UNICAMP; MARRIAM YAMIN, UNICAMP; DANIELLE GAVA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA; MARIA SILVIA VICCARI GATTI, UNICAMP; CARLOS FRANCISCO SAMPAIO BONAFE, UNICAMP; DANIEL FERREIRA DE LIMA NETO, UNICAMP.eng
Aparece nas coleções:Artigo em periódico indexado (CNPSA)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
final9080.pdf2,6 MBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace