Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118368
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dc.contributor.authorDORNELES, E. M. S.eng
dc.contributor.authorFONSECA, M. D. A. M.eng
dc.contributor.authorABREU, J. A. P.eng
dc.contributor.authorLAGE, A. P.eng
dc.contributor.authorBRITO, M. A. V. P.eng
dc.contributor.authorPEREIRA, C. R.eng
dc.contributor.authorBRANDAO, H. de M.eng
dc.contributor.authorGUIMARÃES, A. S.eng
dc.contributor.authorHEINEMANN, M. B.eng
dc.date.accessioned2020-01-08T18:21:47Z-
dc.date.available2020-01-08T18:21:47Z-
dc.date.created2020-01-08
dc.date.issued2019
dc.identifier.citationMicrobiology Open, v. 8, n. 5, article e736, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1118368-
dc.descriptionThe aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed‐field gel electrophoresis (PFGE) genotypes. Seventy‐nine isolates were used, including S. aureus (n = 71) and coagulase‐negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve S. aureus and four CoNS were classified as multidrug resistance strains. Percentage of MRSA was also higher among CoNS (75%), compared to S. aureus isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum‐spanning tree (MST) analysis with data from municipalities, herds, methicillin‐resistant S. aureus (MRSA), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among CoNS, and a high genetic diversity among Staphylococcus spp. isolated from dairy cows with mastitis in Minas Gerais, Brazil.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAntimicrobial resistanceeng
dc.subjectCoagulase-negative staphylococcieng
dc.subjectMethicillin-resistant S aureuseng
dc.subjectPFGEeng
dc.subjectStaphylococcieng
dc.titleGenetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-01-08T18:21:47Z
riaa.ainfo.id1118368eng
riaa.ainfo.lastupdate2020-01-08
dc.identifier.doihttps://doi.org/10.1002/mbo3.736eng
dc.contributor.institutionElaine M. S. Dorneles; Mariana D. A. M. Fonseca; Juliana A. P. Abreu; Andrey P. Lage; Maria A. V. P. Brito; Carine R. Pereira; HUMBERTO DE MELLO BRANDAO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; Marcos B. Heinemann.eng
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