Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121261
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dc.contributor.authorRABELO, C. A. B da S.eng
dc.contributor.authorOKINO, C. H.eng
dc.contributor.authorSAKAMOTO, I. K.eng
dc.contributor.authorVARESCHE, M. B. A.eng
dc.date.accessioned2020-04-03T00:39:44Z-
dc.date.available2020-04-03T00:39:44Z-
dc.date.created2020-03-16
dc.date.issued2020
dc.identifier.citationScience of the Total Environment, v. 715, 2020, 136868.eng
dc.identifier.issn0048-9697eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1121261-
dc.descriptionBioconversion of sugarcane bagasse (SCB) into hydrogen (H2) and organic acids was evaluated using a biomolecular approach to monitor the quantity and expression of the cellulase (Cel) gene. Batch reactors at 37 °C were operated with Paraclostridium sp. (10% v/v) and different substrates (5 g/L): glucose, cellulose and SCB in natura and pre-heat treated and hydrothermally. H2 production from glucose was 162.4 mL via acetic acid (2.9 g/L) and 78.4 mL from cellulose via butyric acid (2.9 g/L). H2 production was higher in hydrothermally pretreated SCB reactors (92.0 mL), heat treated (62.5 mL), when compared to in natura SCB (51.4 mL). Butyric acid (5.8, 4.9 and 4.0 g/L) was the main acid observed in hydrothermally, thermally pretreated, and in natura SCB, respectively. In the reactors with cellulose and reactors with hydrothermally pretreated SCB, the Cel gene copy number 3 and 2 log were higher, respectively, during the stage of maximum H2 production rate, when compared to the initial stage. Differences in Cel gene expression were observed according to the concentration of soluble sugars in the reaction medium. That is, there was no gene expression at the initial phase of the experiment using SCB with 2.6 g/L of sugars and increase of 2.2 log in gene expression during the phases with soluble sugars of <1.4 g/L.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAutochthonous bacteriaeng
dc.subjectQPCReng
dc.subjectReference geneeng
dc.titleIsolation of paraclostridium CR4 from sugarcane bagasse and its evaluation in the bioconversion of lignocellulosic feedstock into hydrogen by monitoring cellulase gene expression.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-04-20T11:11:11Z
dc.subject.nalthesaurusHydrolysiseng
dc.subject.nalthesaurusFermentationeng
dc.format.extent215 p.eng
riaa.ainfo.id1121261eng
riaa.ainfo.lastupdate2020-04-20 -03:00:00
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2020.136868eng
dc.contributor.institutionCAMILA ABREU BORGES DA SILVA RABELO, USP; CINTIA HIROMI OKINO, CPPSE; ISABEL KIMIKO SAKAMOTO, USP; MARIA BERNADETE AMÂNCIO VERESCHE, USP.eng
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