Título:	Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
Autoria:	FAJARDO, T. V. M. Bertocchi, A. A. NICKEL, O.
Afiliação:	THOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV.
Ano de publicação:	2020
Referência:	Revista Ceres, Viçosa, v. 67, n. 2, p. 156-163, mar./apr. 2020.
Conteúdo:	Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey
NAL Thesaurus:	Vitis Pathogens Viruses
Palavras-chave:	RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp)
Digital Object Identifier:	10.1590/0034-737X202067020010
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPUV)