Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124241
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dc.contributor.authorGIACHETTO, P. F.
dc.contributor.authorCUNHA, R. C.
dc.contributor.authorNHANI JUNIOR, A.
dc.contributor.authorGARCIA, M. V.
dc.contributor.authorFERRO, J. A.
dc.contributor.authorANDREOTTI, R.
dc.date.accessioned2020-08-11T11:12:24Z-
dc.date.available2020-08-11T11:12:24Z-
dc.date.created2020-08-10
dc.date.issued2020
dc.identifier.citationFrontiers in Cellular and Infection Microbiology, v. 9, p. 1-15, Jan. 2020.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1124241-
dc.descriptionThe success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectBioinformática
dc.subjectExpressão gênica
dc.subjectRNA-Seq
dc.subjectCattle tick
dc.subjectHost-parasite interaction
dc.titleGene expression in the salivary gland of Rhipicephalus (Boophilus) microplus fed on tick-susceptible and tick-resistant hosts.
dc.typeArtigo de periódico
dc.subject.thesagroGado de Corte
dc.subject.thesagroCarrapato
dc.subject.nalthesaurusSialome
dc.subject.nalthesaurusTranscriptome
dc.subject.nalthesaurusCattle
dc.subject.nalthesaurusBioinformatics
dc.subject.nalthesaurusRhipicephalus microplus
dc.description.notesArticle 477.
riaa.ainfo.id1124241
riaa.ainfo.lastupdate2020-11-06 -02:00:00
dc.identifier.doi10.3389/fcimb.2019.0047
dc.contributor.institutionPOLIANA FERNANDA GIACHETTO, CNPTIA; RODRIGO CASQUERO CUNHA, UFPEL; ANTONIO NHANI JUNIOR, CNPTIA; MARCOS VALERIO GARCIA, CNPGC; JESUS APARECIDO FERRO, UNESP; RENATO ANDREOTTI E SILVA, CNPGC.
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