Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911
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dc.contributor.authorLANA, U. G. de P.
dc.contributor.authorRODRIGUES, M. E. G. de M.
dc.contributor.authorOLIVEIRA-PAIVA, C. A.
dc.contributor.authorSOUSA, S. M. de
dc.contributor.authorTAVARES, A. N. G.
dc.contributor.authorMARRIEL, I. E.
dc.contributor.authorGOMES, E. A.
dc.date.accessioned2020-11-27T09:01:12Z-
dc.date.available2020-11-27T09:01:12Z-
dc.date.created2020-11-22
dc.date.issued2020
dc.identifier.citationRevista Brasileira de Milho e Sorgo, v. 19, e1190, 2020.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911-
dc.descriptionThe use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectPerfil molecular
dc.subjectCrescimento de plantas
dc.titlePolyphasic characterization of bacillus strains isolated from maize.
dc.typeArtigo de periódico
dc.subject.thesagroBactéria
dc.subject.thesagroZea Mays
riaa.ainfo.id1126911
riaa.ainfo.lastupdate2020-11-26
dc.identifier.doihttps://doi.org/10.18512/rbms2020v19e1190
dc.contributor.institutionUBIRACI GOMES DE PAULA LANA, CNPMS
dc.contributor.institutionMARINA ELEUTÉRIO GÓI DE MORAES RODRIGUES, Centro Universitário de Sete Lagoas - UNIFEMMeng
dc.contributor.institutionCHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMSeng
dc.contributor.institutionSYLVIA MORAIS DE SOUSA TINOCO, CNPMSeng
dc.contributor.institutionAMANDA NAYÊ GUIMARÃES TAVARESeng
dc.contributor.institutionIVANILDO EVODIO MARRIEL, CNPMSeng
dc.contributor.institutionELIANE APARECIDA GOMES, CNPMS.eng
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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