Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127907
Título: Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.
Autoria: RODRIGUES, S. A. D.
PONTELO, T. P.
KUSSANO, N. R.
KAWAMOTO, T. S.
LEME, L. O.
CAIXETA, F. M. C.
PFEIFER, L. F. M.
FRANCO, M. M.
DODE, M. A. N.
Afiliação: SARAH A. D. RODRIGUES, Universidade de Brasília
THAIS P. PONTELO, Universidade Federal de Lavras
NAYARA R. KUSSANO, Universidade de Brasília
TAYNAN S. KAWAMOTO, Universidade Federal de Uberlândia
LIGIANE O. LEME, Universidade Federal do Espírito Santo
FELIPPE M. C. CAIXETA, Universidade de Brasília
LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO
MAURICIO MACHAIM FRANCO, Cenargen
MARGOT ALVES NUNES DODE, Cenargen.
Ano de publicação: 2020
Referência: Domestic Animal Endocrinology, v. 72, article 106447, 2020.
Conteúdo: We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes.
Thesagro: Bovinocultura
Reprodução Animal
Embriogénese
Ovulo
Maturação
Cultura In Vitro
Prostaglandina
NAL Thesaurus: Cattle
Animal reproduction
Embryogenesis
Oocytes
In vitro studies
Prostaglandins
Palavras-chave: Oócito
Maturation
E2
F2a
ISSN: 0739-7240
Digital Object Identifier: https://doi.org/10.1016/j.domaniend.2020.106447
Tipo do material: Artigo de periódico
Acesso: openAccess
Aparece nas coleções:Artigo em periódico indexado (CPAF-RO)

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