Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127907
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dc.contributor.authorRODRIGUES, S. A. D.
dc.contributor.authorPONTELO, T. P.
dc.contributor.authorKUSSANO, N. R.
dc.contributor.authorKAWAMOTO, T. S.
dc.contributor.authorLEME, L. O.
dc.contributor.authorCAIXETA, F. M. C.
dc.contributor.authorPFEIFER, L. F. M.
dc.contributor.authorFRANCO, M. M.
dc.contributor.authorDODE, M. A. N.eng
dc.date.accessioned2020-12-10T09:06:38Z-
dc.date.available2020-12-10T09:06:38Z-
dc.date.created2020-12-09
dc.date.issued2020
dc.identifier.citationDomestic Animal Endocrinology, v. 72, article 106447, 2020.
dc.identifier.issn0739-7240
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1127907-
dc.descriptionWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectOócitoeng
dc.subjectMaturationeng
dc.subjectE2eng
dc.subjectF2aeng
dc.titleEffects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.
dc.typeArtigo de periódico
dc.subject.thesagroBovinocultura
dc.subject.thesagroReprodução Animaleng
dc.subject.thesagroEmbriogéneseeng
dc.subject.thesagroOvuloeng
dc.subject.thesagroMaturaçãoeng
dc.subject.thesagroCultura In Vitroeng
dc.subject.thesagroProstaglandinaeng
dc.subject.nalthesaurusCattleeng
dc.subject.nalthesaurusAnimal reproductioneng
dc.subject.nalthesaurusEmbryogenesiseng
dc.subject.nalthesaurusOocyteseng
dc.subject.nalthesaurusIn vitro studieseng
dc.subject.nalthesaurusProstaglandinseng
riaa.ainfo.id1127907
riaa.ainfo.lastupdate2020-12-10 -02:00:00
dc.identifier.doihttps://doi.org/10.1016/j.domaniend.2020.106447
dc.contributor.institutionSARAH A. D. RODRIGUES, Universidade de Brasília
dc.contributor.institutionTHAIS P. PONTELO, Universidade Federal de Lavraseng
dc.contributor.institutionNAYARA R. KUSSANO, Universidade de Brasíliaeng
dc.contributor.institutionTAYNAN S. KAWAMOTO, Universidade Federal de Uberlândiaeng
dc.contributor.institutionLIGIANE O. LEME, Universidade Federal do Espírito Santoeng
dc.contributor.institutionFELIPPE M. C. CAIXETA, Universidade de Brasíliaeng
dc.contributor.institutionLUIZ FRANCISCO MACHADO PFEIFER, CPAF-ROeng
dc.contributor.institutionMAURICIO MACHAIM FRANCO, Cenargeneng
dc.contributor.institutionMARGOT ALVES NUNES DODE, Cenargen.eng
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