Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130630
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dc.contributor.authorVARGAS, D. P.
dc.contributor.authorCORADIN, J. H.
dc.contributor.authorFERREIRA, L. V.
dc.contributor.authorDORNELES, A. O. S.
dc.contributor.authorVALGAS, R. A.
dc.contributor.authorPEREIRA, A. da S.
dc.contributor.authorCASTRO, C. M.
dc.contributor.authorDUTRA, L. F.
dc.date.accessioned2021-03-12T12:01:15Z-
dc.date.available2021-03-12T12:01:15Z-
dc.date.created2021-03-11
dc.date.issued2020
dc.identifier.citationPlant Cell Culture and Micropropagation, v. 16, e155, 2020.
dc.identifier.issn1808-9909
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1130630-
dc.descriptionSynthetic or artificial seeds via encapsulated propagules could be used for in vitro regeneration and mass multiplication, for germplasm preservation and exchange of plant materials. An in vitro encapsulation of potato ?BRS Clara? buds efficient protocol was established. Lateral buds were immersed in a 4% sodium alginate matrix, dripped in CaCl2 for 20 minutes in different osmoregulatory agents: sucrose (8.72, 15.00, 21.28 and 30.00 g L-1) and mannitol (0.00, 11.63, 20.0, 28.37 and 40.0 g L-1). They were then held for 30 days at approximately 4°C and subsequently immersed in KNO3 for capsule decomplexing. Afterward, they were inoculated in MS medium with 3% sucrose and cultured in a growth room under irradiance of 43 μmol m-2 s-1, 16 hours-photoperiod and 25±2 °C temperature. Sucrose and mannitol at concentrations of 15 g L-1 and 20 g L-1, respectively, associated with the encapsulation matrix promoted 80% survival rate of ?BRS Clara? encapsulated buds.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectBRS Clara
dc.titleOsmoregulators on in vitro encapsulation of potato "BRS Clara" buds.
dc.typeArtigo de periódico
dc.subject.thesagroBatata
dc.format.extent26 p.eng
riaa.ainfo.id1130630
riaa.ainfo.lastupdate2021-03-12
dc.contributor.institutionDAIANE PEIXOTO VARGAS
dc.contributor.institutionJULIANA HEY CORADIN, CPACTeng
dc.contributor.institutionLETÍCIA VANNI FERREIRAeng
dc.contributor.institutionATHOS ODIN SEVERO DORNELES, UFPELeng
dc.contributor.institutionRICARDO ALEXANDRE VALGAS, CPACTeng
dc.contributor.institutionARIONE DA SILVA PEREIRA, CPACTeng
dc.contributor.institutionCAROLINE MARQUES CASTRO, CPACTeng
dc.contributor.institutionLEONARDO FERREIRA DUTRA, CPACT.eng
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