Título:	Comparative untargeted metabolome analysis of ruminal fuid and feces of Nelore steers (Bos indicus).
Autoria:	MALHEIROS, J. M. CORREIA, B. S. B. CERIBELI, C. CARDOSO, D. R. COLNAGO, L. A. BOGUSZ JUNIOR, S. REECY, J. M. MOURÃO, G. B. COUTINHO, L. L. PALHARES, J. C. P. BERNDT, A. REGITANO, L. C. de A.
Afiliação:	LUIZ ALBERTO COLNAGO, CNPDIA; JULIO CESAR PASCALE PALHARES, CPPSE; ALEXANDRE BERNDT, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Ano de publicação:	2021
Referência:	Scientific Reports, v. 11, n. 1, e12752, 2021.
Conteúdo:	We conducted a study to identify the fecal metabolite profle and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fuid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented signifcantly higher concentrations of the polar compounds when compared to feces (P< 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fuid metabolites. All fatty acids present in the ruminal fuid were also observed in the feces, except for C20:2n6 and C20:4n6. The identifed metabolites ofer information on the main metabolic pathways (higher impact factor and P< 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The fndings reported herein on the close relationship between the ruminal fuid and feces metabolic profles may ofer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.
Palavras-chave:	Metabolomics analysis Fecal metabolites
ISSN:	2045-2322
Digital Object Identifier:	https://doi.org/10.1038/s41598-021-92179-y
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPDIA)