Título:	In vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants.
Autoria:	SOUZA, J. A. de SANTOS, M. R. A. dos
Afiliação:	JAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
Ano de publicação:	2021
Referência:	International Journal of Development Research, v. 11, n. 12, p. 52392-52393, Dec. 2021.
Conteúdo:	A protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival.
Thesagro:	Reprodução Vegetal Propagação Vegetativa Cultura In Vitro Cultura de Tecido Folha Explante
NAL Thesaurus:	Piper hispidum Plant reproduction Vegetative propagation In vitro culture Tissue culture Somatic embryogenesis Leaves Explants Genetic techniques and protocols
Palavras-chave:	Embriogênese somática Cotyledonary embryos Protocolo de regeneração
ISSN:	2230-9926
Digital Object Identifier:	https://doi.org/10.37118/ijdr.23567.12.2021
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAF-RO)