Título:	Induction of polyploidy in watermelon genotype with powdery mildew resistance (Podosphaera xanthii).
Autoria:	SILVA, C. M. de J. DIAS, R. de C. S. SANTOS, J. S. dos SOUZA, F. de F. MELO, N. F. de
Afiliação:	CARLA MARIA DE JESUS SILVA, Universidade Estadual de Feira de Santana, Feira de Santana, BA; RITA DE CASSIA SOUZA DIAS, CPATSA; JOICE SIMONE DOS SANTOS, Instituto Federal do Sertão Pernambucano, Campus Ouricuri, PE; FLAVIO DE FRANCA SOUZA, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA.
Ano de publicação:	2022
Referência:	Revista Caatinga, v. 35, n. 3, p. 505-513, 2022.
Conteúdo:	Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant.
Thesagro:	Citogenética Citogenética Vegetal Citrullus Lanatus Melancia Doença Oídio
NAL Thesaurus:	Tetraploidy Cytogenetics Watermelons
Palavras-chave:	Podosphaera xanthii Duplicação cromossômica Tetraploidia Melancia triploide
Digital Object Identifier:	http://dx.doi.org/10.1590/1983-21252022v35n301rc
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)