Título:	Phylogenomic and expression analysis of Colossoma macropomum cyp19a1a and cyp19a1b and their non-classical role in tambaqui sex differentiation.
Autoria:	PAIXÃO, R. V. SILVA, G. F. da CAETANO, A. R. CINTRA, L. C. VARELA, E. S. O'SULLIVAN, F. L. A.
Afiliação:	R. V. PAIXÃO, UNIVERSIDADE FEDERAL DO AMAZONAS; GILVAN FERREIRA DA SILVA, CPAA; ALEXANDRE RODRIGUES CAETANO, Cenargen; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.
Ano de publicação:	2022
Referência:	Gene, v. 843, art. 146795, Nov. 2022.
Conteúdo:	The genes coding for Cytochrome P450 aromatase (cyp19a1a and cyp19a1b) and estrogen (E2) receptors (esr1, esr2a and esr2b) play a conserved role in ovarian differentiation and development among teleosts. Classically, the "gonad form" of aromatase, coded by the cyp19a1a, is responsible for the ovarian differentiation in genetic females via ligation and activation of the Esr, which mediates the endocrine and exocrine signaling to allow or block the establishment of the feminine phenotype. However, in neotropical species, studies on the molecular and endocrine processes involved in gonad differentiation as well as on the effects of sex modulators are recent and scarce. In this study, we combined in silico analysis, real-time quantitative PCR (qPCR) assay and quantification of E2 plasma levels of differentiating tambaqui (Colossoma macropomum) to unveil the roles of the paralogs cypa19a1a and cyp19a1b during sex differentiation.
Thesagro:	Tambaqui Colossoma Macropomum
NAL Thesaurus:	Aromatase
Palavras-chave:	Synteny Promoter Structure Sex development Neotropical fish
Digital Object Identifier:	https://doi.org/10.1016/j.gene.2022.146795
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAA)