Título:	Differential gene expression associated with soybean oil level in the diet of pigs.
Autoria:	FINALLI, S. L. SILVA, B. P. M. DA GOMES, J. D. ALMEIDA, V. V. DE FREITAS, F. A. O. MOREIRA, G. C. M. VIGNATO, B. S. AFONSO, J. REECY, J. KOLTES, J. KOLTES, D. REGITANO, L. C. de A. GARRICK, D. J. BALIEIRO, J. C. DE C. MEIRA, A. N. FREITAS, L. COUTINHO, L. L. FUKUMASU, H. MOURÃO, G. B. ALENCAR, S. M. DE LUCHIARI FILHO, A. CESAR, A. S. M.
Afiliação:	SIMARA LARISSA FANALLI, University of São Paulo BRUNA PEREIRA MARTINS DA SILVA, University of São Paulo JULIA DEZEN GOMES, University of São Paulo VIVIAN VEZZONI DE ALMEIDA, Federal University of Goiás FELIPE ANDRÉ OLIVEIRA FREITAS, University of São Paulo GABRIEL COSTA MONTEIRO MOREIRA, University of Liège BÁRBARA SILVA-VIGNATO, University of São Paulo JULIANA AFONSO, University Federal of São Carlos JAMES REECY, Iowa State University JAMES KOLTES, Iowa State University DAWN KOLTES, Iowa State University LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE DORIAN JOHN GARRICK, Massey University JÚLIO CESAR DE CARVALHO BALIEIRO, University of São Paulo ARIANA NASCIMENTO MEIRA, University of São Paulo LUCIANA FREITAS, DB Genética de Suínos LUIZ LEHMANN COUTINHO, University of São Paulo HEIDGE FUKUMASU, University of São Paulo GERSON BARRETO MOURÃO, University of São Paulo SEVERINO MATIAS DE ALENCAR, University of São Paulo ALBINO LUCHIARI FILHO, University of São Paulo ALINE SILVA MELLO CESAR, University of São Paulo.
Ano de publicação:	2022
Referência:	Animals, v. 12, n. 13 1632, jun. 2022.
Páginas:	21 p.
Conteúdo:	The aim of this study was to identify the differentially expressed genes (DEG) from the skeletal muscle and liver samples of animal models for metabolic diseases in humans. To perform the study, the fatty acid (FA) profile and RNA sequencing (RNA-Seq) data of 35 samples of liver tissue (SOY1.5, n = 17 and SOY3.0, n = 18) and 36 samples of skeletal muscle (SOY1.5, n = 18 and SOY3.0, n = 18) of Large White pigs were analyzed. The FA profile of the tissues was modified by the diet, mainly those related to monounsaturated (MUFA) and polyunsaturated (PUFA) FA. The skeletal muscle transcriptome analysis revealed 45 DEG (FDR 10%), and the functional enrichment analysis identified network maps related to inflammation, immune processes, and pathways associated with oxidative stress, type 2 diabetes, and metabolic dysfunction. For the liver tissue, the transcriptome profile analysis revealed 281 DEG, which participate in network maps related to neurodegenerative diseases. With this nutrigenomics study, we verified that different levels of soybean oil in the pig diet, an animal model for metabolic diseases in humans, affected the transcriptome profile of skeletal muscle and liver tissue. These findings may help to better understand the biological mechanisms that can be modulated by the diet.
NAL Thesaurus:	Transcriptome Immune response Metabolism
Palavras-chave:	Fatty acid RNAseq Hepatic tissue Animal model Longissimus lomborum
Digital Object Identifier:	https://doi.org/10.3390/ani12131632
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)