Título:	Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions.
Autoria:	ARRAES, F. B. M. VASQUEZ, D. D. N. TAHIR, M. PINHEIRO, D. H. FAHEEM, M. FREITAS-ALVES, N. S. MOREIRA-PINTO, C. E. MOREIRA, V. J. V. PAES-DE-MELO, B. LISEI-DE-SA, M. E. MORGANTE, C. V. MOTA, A. P. Z. LOURENCO, I. T. TOGAWA, R. C. GRYNBERG, P. FRAGOSO, R. da R. ALMEIDA-ENGLER, J. de LARSEN, M. R. GROSSI-DE-SA, M. F.
Afiliação:	FABRICIO B. M. ARRAES, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL DANIEL D. N. VASQUEZ, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL MUHAMMED TAHIR, UNIVERSITY OF SOUTHERN DENMARK DANIELE H. PINHEIRO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY MUHAMMED FAHEEM, NATIONAL UNIVERSITY OF MEDICAL SCIENCES, PAKISTAN NAYARA S. FREITAS-ALVES, FEDERAL UNIVERSITY OF PARANÁ CLÍDIA E. MOREIRA-PINTO, CNPAE VALDEIR J. V. MOREIRA, UNIVERSITY OF BRASÍLIA BRUNO PAES-DE-MELO, CNPAE MARIA E. LISEI-DE-SA, MINAS GERAIS AGRICULTURAL RESEARCH COMPANY CAROLINA VIANNA MORGANTE, CPATSA ANA P. Z. MOTA, INRAE ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen ROBERTO COITI TOGAWA, Cenargen PRISCILA GRYNBERG, Cenargen RODRIGO DA ROCHA FRAGOSO, CNPAE JANICE DE ALMEIDA-ENGLER, INRAE MARTIN R. LARSEN, UNIVERSITY OF SOUTHERN DENMARK MARIA FATIMA GROSSI-DE-SA, Cenargen.
Ano de publicação:	2022
Referência:	Plants, v. 11, 2744, 2022.
Conteúdo:	The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections.
Thesagro:	Meloidogyne Incognita Soja Glycine Max
NAL Thesaurus:	Transcriptome Proteome Phenylpropanoids
Palavras-chave:	Root-knot nematode Differential expression
ISSN:	2223-7747
Digital Object Identifier:	https:// doi.org/10.3390/plants11202744
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAE)