Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149383
Título: Image-based phenotyping of cassava roots for diversity studies and carotenoids prediction.
Autoria: CARVALHO, R. R. B. de
CORTES, D. F. M.
SOUSA, M. B. e
OLIVEIRA, L. A. de
OLIVEIRA, E. J. de
Afiliação: RAVENA ROCHA BESSA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; DIEGO FERNANDO MARMOLEJO CORTES; MASSAINE BANDEIRA E SOUSA; LUCIANA ALVES DE OLIVEIRA, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF.
Ano de publicação: 2022
Referência: PLoS One, v.17, n.1, e0263326, January, 2022.
Conteúdo: Phenotyping to quantify the total carotenoids content (TCC) is sensitive, time-consuming, tedious, and costly. The development of high-throughput phenotyping tools is essential for screening hundreds of cassava genotypes in a short period of time in the biofortification program. This study aimed to (i) use digital images to extract information on the pulp color of cassava roots and estimate correlations with TCC, and (ii) select predictive models for TCC using colorimetric indices. Red, green and blue images were captured in root samples from 228 biofortified genotypes and the difference in color was analyzed using L*, a*, b*, hue and chroma indices from the International Commission on Illumination (CIELAB) color system and lightness. Colorimetric data were used for principal component analysis (PCA), correlation and for developing prediction models for TCC based on regression and machine learning. A high positive correlation between TCC and the variables b* (r = 0.90) and chroma (r = 0.89) was identified, while the other correlations were median and negative, and the L* parameter did not present a significant correlation with TCC. In general, the accuracy of most prediction models (with all variables and only the most important ones) was high (R2 ranging from 0.81 to 0.94). However, the artificial neural network prediction model presented the best predictive ability (R2 = 0.94), associated with the smallest error in the TCC estimates (root-mean-square error of 0.24). The structure of the studied population revealed five groups and high genetic variability based on PCA regarding colorimetric indices and TCC. Our results demonstrated that the use of data obtained from digital image analysis is an economical, fast, and effective alternative for the development of TCC phenotyping tools in cassava roots with high predictive ability.
Thesagro: Mandioca
ISSN: 1932-6203
Digital Object Identifier: https://doi.org/10.1371/journal.pone.0263326
Tipo do material: Artigo de periódico
Acesso: openAccess
Aparece nas coleções:Artigo em periódico indexado (CNPMF)

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