Título:	Dereplication of sclerotiorin-like azaphilones produced by Penicillium meliponae using LC-MS/MS analysis and molecular networking.
Autoria:	PERES, E. G. SOUZA, M. P. SOUSA, T. F. SILVA, C. V. A. da BARROS, A. L. SILVA, F. M. A. da COSTA, E. V. MEDEIROS, L. S. de FORIM, M. R. SOUZA, A. D. L. de PAZ, W. H. P. SILVA, G. F. da SOUZA, A. Q. L. de KOOLEN, H. H. F.
Afiliação:	ELDRINEI G. PERES, UNIVERSIDADE DO ESTADO DO AMAZONAS; MAYANE P. SOUZA, UNIVERSIDADE FEDERAL DO AMAZONAS; THIAGO F. SOUSA, UNIVERSIDADE DO ESTADO DO AMAZONAS; CARLOS V. A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAZONAS; ANDRÉ L. BARROS, UNIVERSIDADE DO ESTADO DO AMAZONAS; FELIPE M. A. DA SILVA, UNIVERSIDADE FEDERAL DO AMAZONAS; EMMANOEL V. COSTA, UNIVERSIDADE FEDERAL DO AMAZONAS; LÍVIA S. DE MEDEIROS, UNIVERSIDADE FEDERAL DE SÃO PAULO; MOACIR R. FORIM, UNIVERSIDADE FEDERAL DE SÃO CARLOS; AFONSO D. L. DE SOUZA, UNIVERSIDADE FEDERAL DO AMAZONAS; WEIDER H. P. PAZ, UNIVERSIDADE DO ESTADO DO AMAZONAS; GILVAN FERREIRA DA SILVA, CPAA; ANTONIA Q. L. DE SOUZA, UNIVERSIDADE FEDERAL DO AMAZONAS; HECTOR H. F. KOOLEN, UNIVERSIDADE DO ESTADO DO AMAZONAS.
Ano de publicação:	2023
Referência:	Journal of the Brazilian Chemical Society, v. 34, n. 8, p. 1139-1153, 2023.
Conteúdo:	Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones.
Palavras-chave:	Penicillium meliponae Azaphilones Molecular networking
Digital Object Identifier:	https://dx.doi.org/10.21577/0103-5053.20230027
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAA)