Título:	Analyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity.
Autoria:	FAJARDO, T. V. M. GRYNBERG, P. TOGAWA, R. C. SILVA, J. M. F. SILVA, F. N. da NICKEL, O.
Afiliação:	THOR VINICIUS MARTINS FAJARDO, CNPUV; PRISCILA GRYNBERG, Cenargen; ROBERTO COITI TOGAWA, Cenargen; JOÃO MARCOS FAGUNDES SILVA, INSTITUTE FOR INTEGRATIVE SYSTEMS BIOLOGY; FABIO NASCIMENTO DA SILVA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; OSMAR NICKEL, CNPUV.
Ano de publicação:	2024
Referência:	Tropical Plant Pathology, [online], March 2024.
Conteúdo:	The long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses.
NAL Thesaurus:	Closterovirus Ampelovirus Vitivirus
Palavras-chave:	High throughput sequencing Vector
Digital Object Identifier:	https://doi.org/10.1007/s40858-024-00647-3
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPUV)