Título:	Production of wheat starch based artificial endosperm by gel-sandwich technique and plantlet conservion from isolated zygotic embryos in pepper (Capsicum annum Lv. Feherozon).
Autoria:	FARI, M. SAZSZ, A. MITYKÓ, J. MELO, N. F. de
Afiliação:	J. MITYKÓ; NATONIEL FRANKLIN DE MELO, CPATSA.
Ano de publicação:	1997
Referência:	In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 2., 1997, Gramado. Programa e resumos... Passo Fundo: REDBIO Sub-Região Brasil, 1997.
Páginas:	p. 130.
Conteúdo:	Theoretically, the seed-analogue type artificial seed have to be considered as the third generation of the SYNSEED technology. According to this idea, therefore, it should be manufacture this type of artificial seeds with an appropriate artifiical endosperm, which consists of storable, enzymatically digestible and biologically active compounds, to copy the natural seeds. Meanyhile, in the literature of the SYNSEED technology the starch is a neglected compound, however, it is one of the most commonly spread form of storecompound of the endosperm in the seeds of Higher plant. Investigation were carried out in our laboratoy to deveoop appropriate experimental method for the wheat starch, as a potential row material for artificial endosperm. A new method, the "starch gel-sandwich artificial seed" have been established and investigated. Wheat starch based MS (1962) medium was prepared according to the method of Fari (1990), and it was poured into plastic Petri-dishes of 10 cm diameter to make two-millimetre ligh starch-medium layers. Modified pipette-tip was produced from a normal pipette of 100 capacity, which was cul-down at the end to make a cylinder of 8 mm diameter. Wheat starch medium-disks were obtained using this simple instrument with Finpipette. Followint the appropriate embryo-rescue method described by Fari et al. (1983), mature, 32-34 day-old, ring0-shape zygotic embryos of pepper (Capsicum annum L. cv. Feherozon) were isolated and placed onto the surface of the wheat starch medium-disks. After, a second disk was used to form a gel-sandwich structure, consisting one zygotic embryo of pepper and two gel-disks of wheat starch medium. The wsheat start gel-sandwich complexes were dehydrated following the procedure of PVC- foil desiccation method described by Fari et al. (1996), until 50%, 25% and 15% of water content, than they were rehydrated with distilled water. After two weeks, the ratio of germination were claculated and the plantlets were acclimatised and transferred to greenchouse. According to the 50%, 25% ant 15% sater content of the desiccatesd wheat starch gel-sandwiches artificial endosperm, the percentage of the gemination were 100%, 60% and 40%, respectively. The geminated plantlets geminated plantlets germinated well, produced flowers and normal seed set was recorded. On the basis of the "gel-sandwich method", out of whaeat starch, it is possible to test and produce a large scale enzymatically degradable compounds of artificial endosperm, without restriction the characters of chemo-hydrogels, used in the sodium alginate method.
Thesagro:	Embrião Pimentão
NAL Thesaurus:	pepper
Palavras-chave:	Semente sintetica Capsicum annum Synthetic seeds Embryos
Tipo do material:	Resumo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Resumo em anais de congresso (CPATSA)