Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/154317
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dc.contributor.authorAMBROSIO, S. T.pt_BR
dc.contributor.authorMELO, N. F. dept_BR
dc.date.accessioned2017-08-04T23:46:24Z-
dc.date.available2017-08-04T23:46:24Z-
dc.date.created2004-09-07pt_BR
dc.date.issued2004pt_BR
dc.identifier.citationActa Botanica Brasilica, São Paulo, v. 18, n. 4, p. 809-813, 2004.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/154317pt_BR
dc.descriptionInteraction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)). The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60 g.L -1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diam eter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the abs ence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from 15 to 45g.L -1 increased leaf length and diameter and the pH 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. Young sporophytes were successfully acclimated.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPteridófitaspt_BR
dc.subjectCultivo in vitropt_BR
dc.titleInteraction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-09-11T11:11:11Zpt_BR
dc.subject.thesagroCultura de Tecidopt_BR
dc.subject.thesagroPlanta Ornamentalpt_BR
dc.subject.nalthesaurusOrnamental plantspt_BR
dc.subject.nalthesaurusTissue culturept_BR
dc.subject.nalthesaurusNephrolepis biserratapt_BR
riaa.ainfo.id154317pt_BR
riaa.ainfo.lastupdate2018-09-11 -03:00:00pt_BR
dc.contributor.institutionNATONIEL FRANKLIN DE MELO, CPATSA.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CPATSA)

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