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dc.contributor.authorPERECIN, F.pt_BR
dc.contributor.authorNICIURA, S. C. M.pt_BR
dc.contributor.authorYAMAZAKI, W.pt_BR
dc.contributor.authorFERREIRA, C. R.pt_BR
dc.contributor.authorMERIGHE, G. K. F.pt_BR
dc.contributor.authorMEIRELLES, F. V.pt_BR
dc.contributor.authorGARCIA, J. M.eng
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2009-07-30pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/256254pt_BR
dc.descriptionCloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by somatic cell nuclear transfer (SCNT), parthenogenetic activation, and in vitro fertilization (IVF). There was a lower IGF2 expression rate in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to in vivo and IVF embryos (2.01; P < 0.05). In the chorio-allantoic membranes, IGF2 showed a baseline expression pattern (P < 0.05) in parthenotes (0.001) when compared to in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was less expressed (P < 0.05) in SCNT chorio-allantoic membranes (0.25) when compared to the in vivo group. The low expression of IGF2 in parthenogenetic embryos and chorio-allantoic membranes confirms its imprinted status in cattle. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in SCNT-derived bovine embryos and chorio-allantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes of the low efficiency of SCNT procedures in this species.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectIGF2pt_BR
dc.subjectIGF2Rpt_BR
dc.subjectNuclear transferpt_BR
dc.titleImprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-05-10T11:11:11Zpt_BR
dc.subject.nalthesauruscattlept_BR
dc.subject.nalthesaurusepigeneticspt_BR
dc.subject.nalthesaurusgenomic imprintingpt_BR
riaa.ainfo.id256254pt_BR
riaa.ainfo.lastupdate2016-05-10pt_BR
dc.identifier.doi10.4238/vol8-1gmr541eng
dc.contributor.institutionF. PERECIN, FCAV/UNESP, Jaboticabal, SP.pt_BR
dc.contributor.institutionSIMONE CRISTINA MEO NICIURA, CPPSEeng
dc.contributor.institutionW. YAMAZAKI, UNESPeng
dc.contributor.institutionC. R. FERREIRA, FZEA/USP, Pirassununga, SP.eng
dc.contributor.institutionG. K. F. MERIGHE, FZEA/USP, Pirassununga, SP.eng
dc.contributor.institutionF. V. MEIRELLES, FZEA/USP, Pirassununga, SP.eng
dc.contributor.institutionJ. M. GARCIA, FCAV/UNESP, Jaboticabal, SP.eng
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