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dc.contributor.authorPASQUAL, M.pt_BR
dc.contributor.authorDUTRA, L. F.pt_BR
dc.contributor.authorARAUJO, A. G. dept_BR
dc.contributor.authorFIGUEIREDO, M. A. dept_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2009-07-27pt_BR
dc.date.issued2006pt_BR
dc.identifier.citationCiência e Agrotecnologia, Lavras, v. 30, n. 5, p. 1017-1020, set./out. 2006pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/316140pt_BR
dc.descriptionThe aim of this work was to evaluate the effect of Ponkan mandarin (C. reticulata) x Pêra sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (MURASHIGE & SKOOG, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 ?mol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from Ponkan mandarin x Pêra sweet orange embryos inMS medium with 12-18 g L-1 sucrose.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCultura de embriõespt_BR
dc.subjectMeio nutritivopt_BR
dc.titlePonkan mandarin (Citrus reticulata Blanco) immature fruits storage.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-02-25T11:11:11Zpt_BR
dc.subject.thesagroArmazenamentopt_BR
dc.subject.thesagroCitrus Reticulatapt_BR
dc.subject.thesagroMelhoramentopt_BR
dc.subject.thesagroTangerinapt_BR
riaa.ainfo.id316140pt_BR
riaa.ainfo.lastupdate2015-02-25pt_BR
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