Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/484074
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dc.contributor.authorBARDUCHE, D.pt_BR
dc.contributor.authorPAIVA, R.pt_BR
dc.contributor.authorLOPES, M. A.pt_BR
dc.contributor.authorPAIVA, E.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2000-06-26pt_BR
dc.date.issued1999pt_BR
dc.identifier.citationBrazilian Archives of Biology and Technology, Curitiba, v. 42, n. 2, p. 135-144, 1999.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/484074pt_BR
dc.descriptionIn this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectABApt_BR
dc.subjectGA3pt_BR
dc.subjectMobilizacaopt_BR
dc.subjectProtease activitypt_BR
dc.subjectTropical woody plantpt_BR
dc.subjectMobilizationpt_BR
dc.subjectProteinpt_BR
dc.titleEffect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-06-09T11:11:11Zpt_BR
dc.subject.thesagroGerminaçãopt_BR
dc.subject.thesagroProteínapt_BR
dc.subject.nalthesaurusgerminationpt_BR
riaa.ainfo.id484074pt_BR
riaa.ainfo.lastupdate2018-06-09 -03:00:00pt_BR
dc.contributor.institutionEMBRAPA-CNPMS.pt_BR
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