Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/484159
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dc.contributor.authorVALICENTE, F. H.pt_BR
dc.contributor.authorBARRETO, M. R.pt_BR
dc.contributor.authorVASCONCELOS, M. J. V. dept_BR
dc.contributor.authorFIGUEIREDO, J. E. F. dept_BR
dc.contributor.authorPAIVA, E.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2000-09-26pt_BR
dc.date.issued2000pt_BR
dc.identifier.citationAnais da Sociedade Entomológica do Brasil, Jaboticabal, v. 29, n. 1, p. 147-153, 2000.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/484159pt_BR
dc.descriptionFall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectLagarta do cartuchopt_BR
dc.subjectCry1pt_BR
dc.titleIdentificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-06-08T11:11:11Zpt_BR
dc.subject.thesagroControle Biológicopt_BR
dc.subject.thesagroSpodoptera Frugiperdapt_BR
dc.subject.nalthesaurusInsectapt_BR
dc.subject.nalthesaurusbiological controlpt_BR
riaa.ainfo.id484159pt_BR
riaa.ainfo.lastupdate2018-06-08 -03:00:00pt_BR
dc.contributor.institutionFERNANDO HERCOS VALICENTE, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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