Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/490478
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dc.contributor.authorKARAM, D.pt_BR
dc.contributor.authorWESTRA, P.pt_BR
dc.contributor.authorNIESSEN, S. J.pt_BR
dc.contributor.authorWARD, S. M.pt_BR
dc.contributor.authorFIGUEIREDO, J. E. F.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2007-03-22pt_BR
dc.date.issued2006pt_BR
dc.identifier.citationRevista Brasileira de Botânica, São Paulo, v. 29, n. 4, p. 609-615, out./dez. 2006.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/490478pt_BR
dc.descriptionProso millet (Panicum miliaceum) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and 8 primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer combinations were EcoR-AAC/Mse-CTT and EcoR-ACT/Mse-CAA with 7 and 11 polymorphic DNA fragments, respectively. In a total of 450 amplified fragments, at least 339 appeared well separated in a silver stained acrylamide gel and 39 polymorphic DNA bands were scored. The level of polymorphic DNA (11.5%) using only 8 pairs of primers were effective for grouping proso millet biotypes in two clusters but insufficient for separating hybrid biotypes from wild and crop. Nevertheless, the present result indicates that silver stained AFLP markers could be a cheap and important tool for studying genetic relationships in proso millet..pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectDiversidade genéticapt_BR
dc.titleAssessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-05-25T11:11:11Zpt_BR
dc.subject.thesagroMarcador Molecularpt_BR
riaa.ainfo.id490478pt_BR
riaa.ainfo.lastupdate2018-05-25 -03:00:00pt_BR
dc.contributor.institutionDECIO KARAM, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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