Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/65872
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dc.contributor.authorDINIZ, F. M.
dc.contributor.authorIYENGAR, A.
dc.contributor.authorLIMA, P. S. da C.
dc.contributor.authorMACLEAN, N.
dc.contributor.authorBENTZEN, P.
dc.date.accessioned2023-09-14T11:23:43Z-
dc.date.available2023-09-14T11:23:43Z-
dc.date.created2008-03-10
dc.date.issued2007
dc.identifier.citationGenetics and Molecular Biology, São Paulo, v. 30, n. 2, p. 380-384, mar. 2007.
dc.identifier.issn1415-4757
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/65872-
dc.descriptionThe number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic library with an increased proportion of DNA fragments containing repeat motifs. Repeats were found using both types of hybridization but the double-enrichment procedure recovered sequences of which 100% contained (GATA)n and (GACA)n motifs. Microsatellite loci primers were then designed with an M13R-tail or CAG-tag to produce scorable PCR products with minimal stutter. The approach used in this study suggests that double-enrichment is a worthwhile strategy when isolating repeat motifs from eukaryotic genomes. Moreover, the use of tailed microsatellite primers provides increased resolution for compound microsatellite loci, with a significant decrease in costs.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectEnriquecimento duplo
dc.subjectHibridação dupla
dc.subjectBanda polimórfica
dc.subjectMicrossatélite
dc.subjectdouble-enrichment
dc.subjectdouble-hybridization
dc.subjectstutter bands
dc.subjecttailed microsatellite primer
dc.titleApplication of a double-enrichment procedure for microsatellite isolation and the use tailed primers for high throughput genotyping.
dc.typeArtigo de periódico
riaa.ainfo.id65872
riaa.ainfo.lastupdate2023-09-14
dc.contributor.institutionFABIO MENDONCA DINIZ, CPAMN; ARATI IYENGAR; PAULO SARMANHO DA COSTA LIMA, CPAMN; NORMAN MACLEAN; PAUL BENTZEN.
Aparece nas coleções:Artigo em periódico indexado (CPAMN)

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