Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/865928
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dc.contributor.authorGOMES, K. A.pt_BR
dc.contributor.authorALMEIDA, T. C.pt_BR
dc.contributor.authorGESTEIRA, A. da S.pt_BR
dc.contributor.authorLÔBO, I. P.pt_BR
dc.contributor.authorGUIMARÃES, A. C. R.pt_BR
dc.contributor.authorMIRANDA, A. B. dept_BR
dc.contributor.authorVAN SLUYS, M. A.pt_BR
dc.contributor.authorCRUZ, R. S. dapt_BR
dc.contributor.authorCASCARDO, J. C. M.pt_BR
dc.contributor.authorCARELS, N.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2010-11-03pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationGenomics Insights, v. 3, p. 29?56, 2010.pt_BR
dc.identifier.issn1178-6310pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/865928pt_BR
dc.descriptionWe report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBiofuelpt_BR
dc.subjectTerpenespt_BR
dc.subjectPinhão mansopt_BR
dc.subjectMelhoramento genetico vegatalpt_BR
dc.titleESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.thesagroBiocombustívelpt_BR
dc.subject.thesagroJatropha Curcaspt_BR
dc.subject.nalthesaurusalkaloidspt_BR
dc.subject.nalthesaurusfatty acidspt_BR
dc.subject.nalthesaurusgenomicspt_BR
dc.description.notesDiponível em:<http://www.la-press.com/ests-from-seeds-to-assist-the-selective-breeding-of-jatropha-curcas-l--article-a2328>.Acesso em: 03 nov. 2010.pt_BR
riaa.ainfo.id865928pt_BR
riaa.ainfo.lastupdate2010-11-16pt_BR
dc.identifier.doi10.4137/GEI.S4340pt_BR
dc.contributor.institutionKleber A. Gomes, UESC; Tiago C. Almeida, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Ivon Pinheiro Lobo, UESC; Ana Carolina R. Guimarães, FIOCRUZ; Antonio B. de Miranda, FIOCRUZ; Marie-Anne Van Sluys, USP; Rosenira Serpa da Cruz, UESC; Júlio Cézar Mattos Cascardo, UESC; Nicolas Carels, UESC.pt_BR
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